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Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues

Visualization of gene expression at single RNA molecular level represents a great challenge to both imaging technologies and molecular engineering. Here we show a single molecule chromogenic in situ hybridization (smCISH) assay that enables counting and localizing individual RNA molecules in fixed c...

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Detalles Bibliográficos
Autores principales: Jiang, Meng, Liu, Ling, Hong, Chengye, Chen, Debo, Yao, Xihu, Chen, Xiaoyuan, Lin, Chen, Ke, Rongqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6633204/
https://www.ncbi.nlm.nih.gov/pubmed/31064786
http://dx.doi.org/10.1261/rna.070599.119