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Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues
Visualization of gene expression at single RNA molecular level represents a great challenge to both imaging technologies and molecular engineering. Here we show a single molecule chromogenic in situ hybridization (smCISH) assay that enables counting and localizing individual RNA molecules in fixed c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6633204/ https://www.ncbi.nlm.nih.gov/pubmed/31064786 http://dx.doi.org/10.1261/rna.070599.119 |