TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing

Precise genome editing using CRISPR typically requires delivery of guide RNAs, Cas9 endonuclease, and DNA repair templates. Both microinjection and electroporation effectively deliver these components into mouse zygotes provided the DNA template is an oligonucleotide of only a few hundred base pairs...

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Detalles Bibliográficos
Autores principales: Bagheri, Hooman, Friedman, Hana, Shao, Harry, Chong, Yumaine, Lo, Chiu-An, Emran, Farida, Kays, Ibrahim, Yang, Xiang-Jiao, Cooper, Ellis, Chen, Brian E., Siminovitch, Katherine, Peterson, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636866/
https://www.ncbi.nlm.nih.gov/pubmed/31021258
http://dx.doi.org/10.1089/crispr.2017.0020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636866/
https://www.ncbi.nlm.nih.gov/pubmed/31021258
http://dx.doi.org/10.1089/crispr.2017.0020

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