Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro

Notch signaling has been shown to regulate the homeostasis and wound healing of the corneal epithelium. We investigated the effect of Notch inhibition in the human limbal stem/progenitor cells (LSCs) in vitro by using small molecules. Treatment of the LSCs with DAPT and SAHM1 reduced the proliferati...

Descripción completa

Detalles Bibliográficos
Autores principales: González, Sheyla, Uhm, Heui, Deng, Sophie X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637172/
https://www.ncbi.nlm.nih.gov/pubmed/31316119
http://dx.doi.org/10.1038/s41598-019-46793-6
_version_ 1783436188306112512
author González, Sheyla
Uhm, Heui
Deng, Sophie X.
author_facet González, Sheyla
Uhm, Heui
Deng, Sophie X.
author_sort González, Sheyla
collection PubMed
description Notch signaling has been shown to regulate the homeostasis and wound healing of the corneal epithelium. We investigated the effect of Notch inhibition in the human limbal stem/progenitor cells (LSCs) in vitro by using small molecules. Treatment of the LSCs with DAPT and SAHM1 reduced the proliferation rate and maintained the undifferentiated state of the LSCs in a concentration dependent manner. Stratification and differentiation of the corneal epithelium were not reduced after Notch inhibition, indicating that the function of the corneal basal cells is retained. Our findings suggest that Notch signaling plays a role in the proliferation and maintenance of LSCs.
format Online
Article
Text
id pubmed-6637172
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66371722019-07-25 Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro González, Sheyla Uhm, Heui Deng, Sophie X. Sci Rep Article Notch signaling has been shown to regulate the homeostasis and wound healing of the corneal epithelium. We investigated the effect of Notch inhibition in the human limbal stem/progenitor cells (LSCs) in vitro by using small molecules. Treatment of the LSCs with DAPT and SAHM1 reduced the proliferation rate and maintained the undifferentiated state of the LSCs in a concentration dependent manner. Stratification and differentiation of the corneal epithelium were not reduced after Notch inhibition, indicating that the function of the corneal basal cells is retained. Our findings suggest that Notch signaling plays a role in the proliferation and maintenance of LSCs. Nature Publishing Group UK 2019-07-17 /pmc/articles/PMC6637172/ /pubmed/31316119 http://dx.doi.org/10.1038/s41598-019-46793-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
González, Sheyla
Uhm, Heui
Deng, Sophie X.
Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title_full Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title_fullStr Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title_full_unstemmed Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title_short Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro
title_sort notch inhibition prevents differentiation of human limbal stem/progenitor cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637172/
https://www.ncbi.nlm.nih.gov/pubmed/31316119
http://dx.doi.org/10.1038/s41598-019-46793-6
work_keys_str_mv AT gonzalezsheyla notchinhibitionpreventsdifferentiationofhumanlimbalstemprogenitorcellsinvitro
AT uhmheui notchinhibitionpreventsdifferentiationofhumanlimbalstemprogenitorcellsinvitro
AT dengsophiex notchinhibitionpreventsdifferentiationofhumanlimbalstemprogenitorcellsinvitro

Ejemplares similares