A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells

Single-domain antibodies have emerged as highly versatile nanoprobes for advanced cellular imaging. For real-time visualization of endogenous antigens, fluorescently labelled nanobodies (chromobodies, CBs) are introduced as DNA-encoded expression constructs in living cells. Commonly, CB expression i...

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Detalles Bibliográficos
Autores principales: Keller, Bettina-Maria, Maier, Julia, Weldle, Melissa, Segan, Soeren, Traenkle, Bjoern, Rothbauer, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640688/
https://www.ncbi.nlm.nih.gov/pubmed/31544816
http://dx.doi.org/10.3390/antib8010010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640688/
https://www.ncbi.nlm.nih.gov/pubmed/31544816
http://dx.doi.org/10.3390/antib8010010

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