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Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy

Genetic code expansion (GCE) technology allows the specific incorporation of functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the Diels-Alder reaction between trans-cyclooct-2-ene (TCO)-modified ncAAs, and 22 known and novel 1,2,4,5-tetrazine-dye conjugates spanni...

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Detalles Bibliográficos
Autores principales: Beliu, Gerti, Kurz, Andreas J., Kuhlemann, Alexander C., Behringer-Pliess, Lisa, Meub, Mara, Wolf, Natalia, Seibel, Jürgen, Shi, Zhen-Dan, Schnermann, Martin, Grimm, Jonathan B., Lavis, Luke D., Doose, Sören, Sauer, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642216/
https://www.ncbi.nlm.nih.gov/pubmed/31341960
http://dx.doi.org/10.1038/s42003-019-0518-z