Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introd...

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Detalles Bibliográficos
Autores principales: Silva-García, Carlos G., Lanjuin, Anne, Heintz, Caroline, Dutta, Sneha, Clark, Nicole M., Mair, William B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643881/
https://www.ncbi.nlm.nih.gov/pubmed/31064766
http://dx.doi.org/10.1534/g3.119.400314

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643881/
https://www.ncbi.nlm.nih.gov/pubmed/31064766
http://dx.doi.org/10.1534/g3.119.400314

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