Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introd...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva-García, Carlos G., Lanjuin, Anne, Heintz, Caroline, Dutta, Sneha, Clark, Nicole M., Mair, William B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643881/
https://www.ncbi.nlm.nih.gov/pubmed/31064766
http://dx.doi.org/10.1534/g3.119.400314
_version_ 1783437174596698112
author Silva-García, Carlos G.
Lanjuin, Anne
Heintz, Caroline
Dutta, Sneha
Clark, Nicole M.
Mair, William B.
author_facet Silva-García, Carlos G.
Lanjuin, Anne
Heintz, Caroline
Dutta, Sneha
Clark, Nicole M.
Mair, William B.
author_sort Silva-García, Carlos G.
collection PubMed
description We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans.
format Online
Article
Text
id pubmed-6643881
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-66438812019-07-25 Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans Silva-García, Carlos G. Lanjuin, Anne Heintz, Caroline Dutta, Sneha Clark, Nicole M. Mair, William B. G3 (Bethesda) Investigations We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans. Genetics Society of America 2019-05-07 /pmc/articles/PMC6643881/ /pubmed/31064766 http://dx.doi.org/10.1534/g3.119.400314 Text en Copyright © 2019 Silva-Garcia et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Silva-García, Carlos G.
Lanjuin, Anne
Heintz, Caroline
Dutta, Sneha
Clark, Nicole M.
Mair, William B.
Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title_full Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title_fullStr Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title_full_unstemmed Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title_short Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
title_sort single-copy knock-in loci for defined gene expression in caenorhabditis elegans
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643881/
https://www.ncbi.nlm.nih.gov/pubmed/31064766
http://dx.doi.org/10.1534/g3.119.400314
work_keys_str_mv AT silvagarciacarlosg singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans
AT lanjuinanne singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans
AT heintzcaroline singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans
AT duttasneha singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans
AT clarknicolem singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans
AT mairwilliamb singlecopyknockinlocifordefinedgeneexpressionincaenorhabditiselegans

Ejemplares similares