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Silver Nanoparticle Formation-Based Colorimetric Determination of Reducing Sugars in Food Extracts via Tollens’ Reagent
[Image: see text] A simple, sensitive, and nonenzymatic nanospectrophotometric method was developed for the determination of reducing sugars. The silver mirror reaction-assisted method is based on the in situ formation of silver nanoparticles in the presence of reducing sugars. All simple reducing s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647951/ https://www.ncbi.nlm.nih.gov/pubmed/31459853 http://dx.doi.org/10.1021/acsomega.9b00761 |
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author | Durmazel, Selen Üzer, Ayşem Erbil, Buse Sayın, Buse Apak, Reşat |
author_facet | Durmazel, Selen Üzer, Ayşem Erbil, Buse Sayın, Buse Apak, Reşat |
author_sort | Durmazel, Selen |
collection | PubMed |
description | [Image: see text] A simple, sensitive, and nonenzymatic nanospectrophotometric method was developed for the determination of reducing sugars. The silver mirror reaction-assisted method is based on the in situ formation of silver nanoparticles in the presence of reducing sugars. All simple reducing sugars (glucose, galactose, fructose, mannose, maltose, and lactose) examined had perfectly linear regression equations. The detection limit for glucose was 40 nM. The proposed method could be selectively applied to various synthetic mixtures of reducing sugars with polyphenolic compounds, and to honey, milk, and commercial fruit juice as real samples using solid phase extraction as a clean-up process. The developed method was also statistically validated against conventional alkaline CUPRAC (cupric–neocuproine, Cu(II)–Nc) spectrophotometric method using Student’s t- and F-tests. |
format | Online Article Text |
id | pubmed-6647951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-66479512019-08-27 Silver Nanoparticle Formation-Based Colorimetric Determination of Reducing Sugars in Food Extracts via Tollens’ Reagent Durmazel, Selen Üzer, Ayşem Erbil, Buse Sayın, Buse Apak, Reşat ACS Omega [Image: see text] A simple, sensitive, and nonenzymatic nanospectrophotometric method was developed for the determination of reducing sugars. The silver mirror reaction-assisted method is based on the in situ formation of silver nanoparticles in the presence of reducing sugars. All simple reducing sugars (glucose, galactose, fructose, mannose, maltose, and lactose) examined had perfectly linear regression equations. The detection limit for glucose was 40 nM. The proposed method could be selectively applied to various synthetic mixtures of reducing sugars with polyphenolic compounds, and to honey, milk, and commercial fruit juice as real samples using solid phase extraction as a clean-up process. The developed method was also statistically validated against conventional alkaline CUPRAC (cupric–neocuproine, Cu(II)–Nc) spectrophotometric method using Student’s t- and F-tests. American Chemical Society 2019-04-25 /pmc/articles/PMC6647951/ /pubmed/31459853 http://dx.doi.org/10.1021/acsomega.9b00761 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Durmazel, Selen Üzer, Ayşem Erbil, Buse Sayın, Buse Apak, Reşat Silver Nanoparticle Formation-Based Colorimetric Determination of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title | Silver Nanoparticle Formation-Based Colorimetric Determination
of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title_full | Silver Nanoparticle Formation-Based Colorimetric Determination
of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title_fullStr | Silver Nanoparticle Formation-Based Colorimetric Determination
of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title_full_unstemmed | Silver Nanoparticle Formation-Based Colorimetric Determination
of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title_short | Silver Nanoparticle Formation-Based Colorimetric Determination
of Reducing Sugars in Food Extracts via Tollens’ Reagent |
title_sort | silver nanoparticle formation-based colorimetric determination
of reducing sugars in food extracts via tollens’ reagent |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647951/ https://www.ncbi.nlm.nih.gov/pubmed/31459853 http://dx.doi.org/10.1021/acsomega.9b00761 |
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