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A collagen-based microwell migration assay to study NK-target cell interactions
Natural killer (NK) cell cytotoxicity in tissue is dependent on the ability of NK cells to migrate through the extracellular matrix (ECM) microenvironment. Traditional imaging studies of NK cell migration and cytotoxicity have utilized 2D surfaces, which do not properly reproduce the structural and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650390/ https://www.ncbi.nlm.nih.gov/pubmed/31337806 http://dx.doi.org/10.1038/s41598-019-46958-3 |
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author | Olofsson, Per E. Brandt, Ludwig Magnusson, Klas E. G. Frisk, Thomas Jaldén, Joakim Önfelt, Björn |
author_facet | Olofsson, Per E. Brandt, Ludwig Magnusson, Klas E. G. Frisk, Thomas Jaldén, Joakim Önfelt, Björn |
author_sort | Olofsson, Per E. |
collection | PubMed |
description | Natural killer (NK) cell cytotoxicity in tissue is dependent on the ability of NK cells to migrate through the extracellular matrix (ECM) microenvironment. Traditional imaging studies of NK cell migration and cytotoxicity have utilized 2D surfaces, which do not properly reproduce the structural and mechanical cues that shape the migratory response of NK cells in vivo. Here, we have combined a microwell assay that allows long-term imaging and tracking of small, well-defined populations of NK cells with an interstitial ECM-like matrix. The assay allows for long-term imaging of NK–target cell interactions within a confined 3D volume. We found marked differences in motility between individual cells with a small fraction of the cells moving slowly and being confined to a small volume within the matrix, while other cells moved more freely. A majority of NK cells also exhibited transient variation in their motility, alternating between periods of migration arrest and movement. The assay could be used as a complement to in vivo imaging to study human NK cell heterogeneity in migration and cytotoxicity. |
format | Online Article Text |
id | pubmed-6650390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66503902019-07-29 A collagen-based microwell migration assay to study NK-target cell interactions Olofsson, Per E. Brandt, Ludwig Magnusson, Klas E. G. Frisk, Thomas Jaldén, Joakim Önfelt, Björn Sci Rep Article Natural killer (NK) cell cytotoxicity in tissue is dependent on the ability of NK cells to migrate through the extracellular matrix (ECM) microenvironment. Traditional imaging studies of NK cell migration and cytotoxicity have utilized 2D surfaces, which do not properly reproduce the structural and mechanical cues that shape the migratory response of NK cells in vivo. Here, we have combined a microwell assay that allows long-term imaging and tracking of small, well-defined populations of NK cells with an interstitial ECM-like matrix. The assay allows for long-term imaging of NK–target cell interactions within a confined 3D volume. We found marked differences in motility between individual cells with a small fraction of the cells moving slowly and being confined to a small volume within the matrix, while other cells moved more freely. A majority of NK cells also exhibited transient variation in their motility, alternating between periods of migration arrest and movement. The assay could be used as a complement to in vivo imaging to study human NK cell heterogeneity in migration and cytotoxicity. Nature Publishing Group UK 2019-07-23 /pmc/articles/PMC6650390/ /pubmed/31337806 http://dx.doi.org/10.1038/s41598-019-46958-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Olofsson, Per E. Brandt, Ludwig Magnusson, Klas E. G. Frisk, Thomas Jaldén, Joakim Önfelt, Björn A collagen-based microwell migration assay to study NK-target cell interactions |
title | A collagen-based microwell migration assay to study NK-target cell interactions |
title_full | A collagen-based microwell migration assay to study NK-target cell interactions |
title_fullStr | A collagen-based microwell migration assay to study NK-target cell interactions |
title_full_unstemmed | A collagen-based microwell migration assay to study NK-target cell interactions |
title_short | A collagen-based microwell migration assay to study NK-target cell interactions |
title_sort | collagen-based microwell migration assay to study nk-target cell interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650390/ https://www.ncbi.nlm.nih.gov/pubmed/31337806 http://dx.doi.org/10.1038/s41598-019-46958-3 |
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