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A standard for near-scarless plasmid construction using reusable DNA parts

Here we report GT (Guanin/Thymine) standard (GTS) for plasmid construction under which DNA sequences are defined as two types of standard, reusable parts (fragment and barcode). We develop a technology that can efficiently add any two barcodes to two ends of any fragment without leaving scars in mos...

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Detalles Bibliográficos
Autores principales: Ma, Xiaoqiang, Liang, Hong, Cui, Xiaoyi, Liu, Yurou, Lu, Hongyuan, Ning, Wenbo, Poon, Nga Yu, Ho, Benjamin, Zhou, Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650416/
https://www.ncbi.nlm.nih.gov/pubmed/31337759
http://dx.doi.org/10.1038/s41467-019-11263-0