Cargando…

mRNA Engineering for the Efficient Chaperone-Mediated Co-Translational Folding of Recombinant Proteins in Escherichia coli

The production of soluble, functional recombinant proteins by engineered bacterial hosts is challenging. Natural molecular chaperone systems have been used to solubilize various recombinant proteins with limited success. Here, we attempted to facilitate chaperone-mediated folding by directing the mo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bui, Le Minh, Geraldi, Almando, Nguyen, Thi Thuy, Lee, Jun Hyoung, Lee, Ju Young, Cho, Byung-Kwan, Kim, Sun Chang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651523/ https://www.ncbi.nlm.nih.gov/pubmed/31261687 http://dx.doi.org/10.3390/ijms20133163

Ejemplares similares

Enhanced Production of Fatty Acid Ethyl Ester with Engineered fabHDG Operon in Escherichia coli
por: Rahman, Ziaur, et al.
Publicado: (2019)

Synthetic Scaffold Systems for Increasing the Efficiency of Metabolic Pathways in Microorganisms
por: Geraldi, Almando, et al.
Publicado: (2021)

Exploring the Potential of a Genome-Reduced Escherichia coli Strain for Plasmid DNA Production
por: Nguyen, Thi Thuy, et al.
Publicado: (2023)

Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of Escherichia coli()
por: O'Reilly, A.O., et al.
Publicado: (2014)

Production of Indigo by Recombinant Escherichia coli with Expression of Monooxygenase, Tryptophanase, and Molecular Chaperone
por: Du, Lingyan, et al.
Publicado: (2022)

Chaperone-Usher Fimbriae of Escherichia coli
por: Wurpel, Daniël J., et al.
Publicado: (2013)

Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli
por: Gawin, Agnieszka, et al.
Publicado: (2020)

Update on the Development of Toehold Switch-Based Approach for Molecular Diagnostic Tests of COVID-19
por: Geraldi, Almando, et al.
Publicado: (2022)

Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli
por: Pang, Cuiping, et al.
Publicado: (2022)

An overview on molecular chaperones enhancing solubility of expressed recombinant proteins with correct folding
por: Mamipour, Mina, et al.
Publicado: (2017)

AP profiling resolves co-translational folding pathway and chaperone interactions in vivo
por: Chen, Xiuqi, et al.
Publicado: (2023)

Direct observation of tRNA-chaperoned folding of a dynamic mRNA ensemble
por: Suddala, Krishna C., et al.
Publicado: (2023)

Influence of Escherichia coli chaperone DnaK on protein immunogenicity
por: Ratanji, Kirsty D., et al.
Publicado: (2016)

Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli
por: Elfageih, Rageia, et al.
Publicado: (2020)

Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid
por: Quyen, Thi Dinh, et al.
Publicado: (2012)

Synergistic Regulation of Transcription and Translation in Escherichia coli Revealed by Codirectional Increases in mRNA Concentration and Translation Efficiency
por: Nguyen, Huong Le, et al.
Publicado: (2022)

Visualizing chaperone-assisted protein folding
por: Horowitz, Scott, et al.
Publicado: (2016)

Genome engineering for improved recombinant protein expression in Escherichia coli
por: Mahalik, Shubhashree, et al.
Publicado: (2014)

PpiD is a player in the network of periplasmic chaperones in Escherichia coli
por: Matern, Yvonne, et al.
Publicado: (2010)

Determination of the intracellular concentration of the export chaperone SecB in Escherichia coli
por: Findik, Bahar T., et al.
Publicado: (2017)

Adaptive laboratory evolution of a genome-reduced Escherichia coli
por: Choe, Donghui, et al.
Publicado: (2019)

Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins in Escherichia coli: effect of free DnaK level on temperature-induced recombinant streptokinase production
por: Balagurunathan, Balaji, et al.
Publicado: (2009)

Effect of Signal Peptide on Stability and Folding of Escherichia coli Thioredoxin
por: Singh, Pranveer, et al.
Publicado: (2013)

Protein folding while chaperone bound is dependent on weak interactions
por: Wu, Kevin, et al.
Publicado: (2019)

Enhancement of the Chaperone Activity of Alkyl Hydroperoxide Reductase C from Pseudomonas aeruginosa PAO1 Resulting from a Point-Specific Mutation Confers Heat Tolerance in Escherichia coli
por: Lee, Jae Taek, et al.
Publicado: (2016)

Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding
por: Chen, Yan, et al.
Publicado: (2022)

Visualized and precise design of artificial small RNAs for regulating T7 RNA polymerase and enhancing recombinant protein folding in Escherichia coli
por: Zhao, Yujia, et al.
Publicado: (2016)

Regulation of chaperone function by coupled folding and oligomerization
por: Mas, Guillaume, et al.
Publicado: (2020)

Formation of Fibrils by the Periplasmic Molecular Chaperone HdeB from Escherichia coli
por: Nakata, Yui, et al.
Publicado: (2022)

Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli
por: Lee, Ju Young, et al.
Publicado: (2008)

Attractor Concepts to Evaluate the Transcriptome-wide Dynamics Guiding Anaerobic to Aerobic State Transition in Escherichia coli
por: Bui, Thuy Tien, et al.
Publicado: (2020)

MazF activation promotes translational heterogeneity of the grcA mRNA in Escherichia coli populations
por: Nikolic, Nela, et al.
Publicado: (2017)

An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli
por: Romilly, Cédric, et al.
Publicado: (2020)

Exploration of twin‐arginine translocation for expression and purification of correctly folded proteins in Escherichia coli
por: Fisher, Adam C., et al.
Publicado: (2008)

Use of folding modulators to improve heterologous protein production in Escherichia coli
por: Kolaj, Olga, et al.
Publicado: (2009)

Heat and Pressure Resistance in Escherichia coli Relates to Protein Folding and Aggregation
por: Li, Hui, et al.
Publicado: (2020)

A quantitative interpretation of oxidative protein folding activity in Escherichia coli
por: Rettenbacher, Lukas A., et al.
Publicado: (2022)

Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis
por: Haeger, Gerrit, et al.
Publicado: (2023)

Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in Escherichia coli
por: Kim, Jaoon YH, et al.
Publicado: (2010)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_7nti0ojcsqc2qdp926qmbsl8r9