2-Ketogluconate Kinase from Cupriavidus necator H16: Purification, Characterization, and Exploration of Its Substrate Specificity

We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from Cupriavidus necator (Ralstonia eutropha) H16. Exploration of its substrate specificity revealed that three ketoacids (2-keto-3-deoxy-d-gluconate, 2-keto-d-gulonate, and 2-ke...

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Detalles Bibliográficos
Autores principales: Sánchez-Moreno, Israel, Trachtmann, Natalia, Ilhan, Sibel, Hélaine, Virgil, Lemaire, Marielle, Guérard-Hélaine, Christine, Sprenger, Georg A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651773/
https://www.ncbi.nlm.nih.gov/pubmed/31261738
http://dx.doi.org/10.3390/molecules24132393

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651773/
https://www.ncbi.nlm.nih.gov/pubmed/31261738
http://dx.doi.org/10.3390/molecules24132393

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