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2-Ketogluconate Kinase from Cupriavidus necator H16: Purification, Characterization, and Exploration of Its Substrate Specificity
We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from Cupriavidus necator (Ralstonia eutropha) H16. Exploration of its substrate specificity revealed that three ketoacids (2-keto-3-deoxy-d-gluconate, 2-keto-d-gulonate, and 2-ke...
Autores principales: | Sánchez-Moreno, Israel, Trachtmann, Natalia, Ilhan, Sibel, Hélaine, Virgil, Lemaire, Marielle, Guérard-Hélaine, Christine, Sprenger, Georg A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651773/ https://www.ncbi.nlm.nih.gov/pubmed/31261738 http://dx.doi.org/10.3390/molecules24132393 |
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