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Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BACKGROUND: Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe technology from approximately 150 bps to sev...

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Detalles Bibliográficos
Autores principales: Shukor, Syukri, Tamayo, Alfred, Tosi, Lorenzo, Larman, H. Benjamin, Parekkadan, Biju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657055/
https://www.ncbi.nlm.nih.gov/pubmed/31340783
http://dx.doi.org/10.1186/s12896-019-0547-1