Cargando…
Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
BACKGROUND: Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe technology from approximately 150 bps to sev...
Autores principales: | Shukor, Syukri, Tamayo, Alfred, Tosi, Lorenzo, Larman, H. Benjamin, Parekkadan, Biju |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657055/ https://www.ncbi.nlm.nih.gov/pubmed/31340783 http://dx.doi.org/10.1186/s12896-019-0547-1 |
Ejemplares similares
-
Computational Simulation of Adapter Length-Dependent LASSO Probe Capture Efficiency
por: Liu, Jingqian, et al.
Publicado: (2019) -
Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
por: Tosi, Lorenzo, et al.
Publicado: (2017) -
Tracking leukemic T‐cell transcriptional dynamics in vivo with a blood‐based reporter assay
por: Tamayo, Alfred G., et al.
Publicado: (2020) -
Quantitative and multiplexed DNA methylation analysis using long-read single-molecule real-time bisulfite sequencing (SMRT-BS)
por: Yang, Yao, et al.
Publicado: (2015) -
Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg
por: Bordat, Amandine, et al.
Publicado: (2015)