Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo

The CRISPR/Cas9 system is unable to edit all targetable genomic sites with full efficiency in vivo. We show that Cas9-mediated editing is more efficient in open chromatin regions than in closed chromatin regions in rice. A construct (Cas9-TV) formed by fusing a synthetic transcription activation dom...

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Detalles Bibliográficos
Autores principales: Liu, Guanwen, Yin, Kangquan, Zhang, Qianwei, Gao, Caixia, Qiu, Jin-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660936/
https://www.ncbi.nlm.nih.gov/pubmed/31349852
http://dx.doi.org/10.1186/s13059-019-1762-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660936/
https://www.ncbi.nlm.nih.gov/pubmed/31349852
http://dx.doi.org/10.1186/s13059-019-1762-8

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