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Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo
The CRISPR/Cas9 system is unable to edit all targetable genomic sites with full efficiency in vivo. We show that Cas9-mediated editing is more efficient in open chromatin regions than in closed chromatin regions in rice. A construct (Cas9-TV) formed by fusing a synthetic transcription activation dom...
Autores principales: | Liu, Guanwen, Yin, Kangquan, Zhang, Qianwei, Gao, Caixia, Qiu, Jin-Long |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660936/ https://www.ncbi.nlm.nih.gov/pubmed/31349852 http://dx.doi.org/10.1186/s13059-019-1762-8 |
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