Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro

[Image: see text] Here, we report that wild type Escherichia coli ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-pept...

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Autores principales: Ad, Omer, Hoffman, Kyle S., Cairns, Andrew G., Featherston, Aaron L., Miller, Scott J., Söll, Dieter, Schepartz, Alanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6661870/
https://www.ncbi.nlm.nih.gov/pubmed/31403077
http://dx.doi.org/10.1021/acscentsci.9b00460
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author Ad, Omer
Hoffman, Kyle S.
Cairns, Andrew G.
Featherston, Aaron L.
Miller, Scott J.
Söll, Dieter
Schepartz, Alanna
author_facet Ad, Omer
Hoffman, Kyle S.
Cairns, Andrew G.
Featherston, Aaron L.
Miller, Scott J.
Söll, Dieter
Schepartz, Alanna
author_sort Ad, Omer
collection PubMed
description [Image: see text] Here, we report that wild type Escherichia coli ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-peptide hybrid molecules. This work expands the scope of ribozyme- and ribosome-catalyzed chemical transformations, provides a starting point for in vivo translation engineering efforts, and offers an alternative strategy for the biosynthesis of polyketide-peptide natural products.
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spelling pubmed-66618702019-08-09 Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro Ad, Omer Hoffman, Kyle S. Cairns, Andrew G. Featherston, Aaron L. Miller, Scott J. Söll, Dieter Schepartz, Alanna ACS Cent Sci [Image: see text] Here, we report that wild type Escherichia coli ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-peptide hybrid molecules. This work expands the scope of ribozyme- and ribosome-catalyzed chemical transformations, provides a starting point for in vivo translation engineering efforts, and offers an alternative strategy for the biosynthesis of polyketide-peptide natural products. American Chemical Society 2019-06-26 2019-07-24 /pmc/articles/PMC6661870/ /pubmed/31403077 http://dx.doi.org/10.1021/acscentsci.9b00460 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Ad, Omer
Hoffman, Kyle S.
Cairns, Andrew G.
Featherston, Aaron L.
Miller, Scott J.
Söll, Dieter
Schepartz, Alanna
Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title_full Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title_fullStr Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title_full_unstemmed Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title_short Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
title_sort translation of diverse aramid- and 1,3-dicarbonyl-peptides by wild type ribosomes in vitro
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6661870/
https://www.ncbi.nlm.nih.gov/pubmed/31403077
http://dx.doi.org/10.1021/acscentsci.9b00460
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