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Activity‐based proteomics reveals nine target proteases for the recombinant protein‐stabilizing inhibitor Sl CYS8 in Nicotiana benthamiana

Co‐expression of protease inhibitors like the tomato cystatin Sl CYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity‐based protein profiling to identify proteases that are inhibited by S...

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Detalles Bibliográficos
Autores principales: Jutras, Philippe V., Grosse‐Holz, Friederike, Kaschani, Farnusch, Kaiser, Markus, Michaud, Dominique, van der Hoorn, Renier A.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662110/
https://www.ncbi.nlm.nih.gov/pubmed/30742730
http://dx.doi.org/10.1111/pbi.13092