Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design

Site-directed methods for the generation of genetic diversity are essential tools in the field of directed enzyme evolution. The Golden Gate cloning technique has been proven to be an efficient tool for a variety of cloning setups. The utilization of restriction enzymes which cut outside of their re...

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Detalles Bibliográficos
Autores principales: Püllmann, Pascal, Ulpinnis, Chris, Marillonnet, Sylvestre, Gruetzner, Ramona, Neumann, Steffen, Weissenborn, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662682/
https://www.ncbi.nlm.nih.gov/pubmed/31358887
http://dx.doi.org/10.1038/s41598-019-47376-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662682/
https://www.ncbi.nlm.nih.gov/pubmed/31358887
http://dx.doi.org/10.1038/s41598-019-47376-1

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