Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos

Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In the present study, we aimed to investigate the effect of sodium butyrate (NaBu), a natural histone deacetylase inhibitor (HDACi), on the maturat...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Meng-Fei, Wang, Ju-Long, Yi, Jian-Ming, Ma, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663018/
https://www.ncbi.nlm.nih.gov/pubmed/31356635
http://dx.doi.org/10.1371/journal.pone.0220479
_version_ 1783439751746945024
author Yu, Meng-Fei
Wang, Ju-Long
Yi, Jian-Ming
Ma, Lin
author_facet Yu, Meng-Fei
Wang, Ju-Long
Yi, Jian-Ming
Ma, Lin
author_sort Yu, Meng-Fei
collection PubMed
description Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In the present study, we aimed to investigate the effect of sodium butyrate (NaBu), a natural histone deacetylase inhibitor (HDACi), on the maturation of oocytes, preimplantation embryonic development, and expression of important developmental genes. The results indicated that NaBu decreased the rates of GVBD and the first polar body extrusion (PBE) in vitro in a dose-dependent manner. Meanwhile, NaBu treatment led to an abnormality in the spindle apparatus in oocytes in MI. However, the ratio of phosphor-extracellular signal-regulated kinases (p-ERK)/ERK significantly decreased in oocytes treated with 2.0 mM NaBu for 8 h. Furthermore, NaBu treatment at 2.0 mM improved the quality of embryos and the mRNA expression levels of important developmental genes such as HDAC1, Sox2, and Pou5f1. These data suggest that although a high concentration NaBu will impede the meiosis of oocytes, 2.0 mM NaBu will promote the development of embryos in vitro. Further investigation is needed to clarify the direct/indirect effects of NaBu on the regulation of important developmental genes and their subsequent impacts on full-term development in mammals.
format Online
Article
Text
id pubmed-6663018
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-66630182019-08-07 Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos Yu, Meng-Fei Wang, Ju-Long Yi, Jian-Ming Ma, Lin PLoS One Research Article Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In the present study, we aimed to investigate the effect of sodium butyrate (NaBu), a natural histone deacetylase inhibitor (HDACi), on the maturation of oocytes, preimplantation embryonic development, and expression of important developmental genes. The results indicated that NaBu decreased the rates of GVBD and the first polar body extrusion (PBE) in vitro in a dose-dependent manner. Meanwhile, NaBu treatment led to an abnormality in the spindle apparatus in oocytes in MI. However, the ratio of phosphor-extracellular signal-regulated kinases (p-ERK)/ERK significantly decreased in oocytes treated with 2.0 mM NaBu for 8 h. Furthermore, NaBu treatment at 2.0 mM improved the quality of embryos and the mRNA expression levels of important developmental genes such as HDAC1, Sox2, and Pou5f1. These data suggest that although a high concentration NaBu will impede the meiosis of oocytes, 2.0 mM NaBu will promote the development of embryos in vitro. Further investigation is needed to clarify the direct/indirect effects of NaBu on the regulation of important developmental genes and their subsequent impacts on full-term development in mammals. Public Library of Science 2019-07-29 /pmc/articles/PMC6663018/ /pubmed/31356635 http://dx.doi.org/10.1371/journal.pone.0220479 Text en © 2019 Yu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yu, Meng-Fei
Wang, Ju-Long
Yi, Jian-Ming
Ma, Lin
Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title_full Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title_fullStr Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title_full_unstemmed Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title_short Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
title_sort sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663018/
https://www.ncbi.nlm.nih.gov/pubmed/31356635
http://dx.doi.org/10.1371/journal.pone.0220479
work_keys_str_mv AT yumengfei sodiumbutyrateinterruptsthematurationofoocytesandenhancesthedevelopmentofpreimplantationembryos
AT wangjulong sodiumbutyrateinterruptsthematurationofoocytesandenhancesthedevelopmentofpreimplantationembryos
AT yijianming sodiumbutyrateinterruptsthematurationofoocytesandenhancesthedevelopmentofpreimplantationembryos
AT malin sodiumbutyrateinterruptsthematurationofoocytesandenhancesthedevelopmentofpreimplantationembryos