Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation

Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE...

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Detalles Bibliográficos
Autores principales: Machado, Rosa, Bendesky, Justin, Brown, Madison, Spendier, Kathrin, Hagen, Guy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663088/
https://www.ncbi.nlm.nih.gov/pubmed/31360699
http://dx.doi.org/10.3390/jimaging5070063

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663088/
https://www.ncbi.nlm.nih.gov/pubmed/31360699
http://dx.doi.org/10.3390/jimaging5070063

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