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The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome

Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of transcriptomes, especially for complex genomes c...

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Detalles Bibliográficos
Autores principales: Hoang, Nam V., Furtado, Agnelo, Perlo, Virginie, Botha, Frederik C., Henry, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664245/
https://www.ncbi.nlm.nih.gov/pubmed/31396260
http://dx.doi.org/10.3389/fgene.2019.00654