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The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome
Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of transcriptomes, especially for complex genomes c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664245/ https://www.ncbi.nlm.nih.gov/pubmed/31396260 http://dx.doi.org/10.3389/fgene.2019.00654 |