Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

Proteins that contain disulfide bonds mainly mature in the oxidative environment of the eukaryotic endoplasmic reticulum or the periplasm of Gram-negative bacteria. In E. coli, disulfide bond containing recombinant proteins are often targeted to the periplasm by an N-terminal signal peptide that is...

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Detalles Bibliográficos
Autores principales: Karyolaimos, Alexandros, Ampah-Korsah, Henry, Hillenaar, Tamara, Mestre Borras, Anna, Dolata, Katarzyna Magdalena, Sievers, Susanne, Riedel, Katharina, Daniels, Robert, de Gier, Jan-Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664373/
https://www.ncbi.nlm.nih.gov/pubmed/31396164
http://dx.doi.org/10.3389/fmicb.2019.01511

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664373/
https://www.ncbi.nlm.nih.gov/pubmed/31396164
http://dx.doi.org/10.3389/fmicb.2019.01511

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