Does the mouse tail vein injection method provide a good model of lung cancer?

Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of...

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Autores principales: Shrestha, Nensi, Lateef, Zabeen, Martey, Orleans, Bland, Abigail R., Nimick, Mhairi, Rosengren, Rhonda, Ashton, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668049/
https://www.ncbi.nlm.nih.gov/pubmed/31448098
http://dx.doi.org/10.12688/f1000research.17964.1
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author Shrestha, Nensi
Lateef, Zabeen
Martey, Orleans
Bland, Abigail R.
Nimick, Mhairi
Rosengren, Rhonda
Ashton, John C.
author_facet Shrestha, Nensi
Lateef, Zabeen
Martey, Orleans
Bland, Abigail R.
Nimick, Mhairi
Rosengren, Rhonda
Ashton, John C.
author_sort Shrestha, Nensi
collection PubMed
description Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line.
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spelling pubmed-66680492019-08-22 Does the mouse tail vein injection method provide a good model of lung cancer? Shrestha, Nensi Lateef, Zabeen Martey, Orleans Bland, Abigail R. Nimick, Mhairi Rosengren, Rhonda Ashton, John C. F1000Res Research Article Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line. F1000 Research Limited 2019-02-15 /pmc/articles/PMC6668049/ /pubmed/31448098 http://dx.doi.org/10.12688/f1000research.17964.1 Text en Copyright: © 2019 Shrestha N et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shrestha, Nensi
Lateef, Zabeen
Martey, Orleans
Bland, Abigail R.
Nimick, Mhairi
Rosengren, Rhonda
Ashton, John C.
Does the mouse tail vein injection method provide a good model of lung cancer?
title Does the mouse tail vein injection method provide a good model of lung cancer?
title_full Does the mouse tail vein injection method provide a good model of lung cancer?
title_fullStr Does the mouse tail vein injection method provide a good model of lung cancer?
title_full_unstemmed Does the mouse tail vein injection method provide a good model of lung cancer?
title_short Does the mouse tail vein injection method provide a good model of lung cancer?
title_sort does the mouse tail vein injection method provide a good model of lung cancer?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668049/
https://www.ncbi.nlm.nih.gov/pubmed/31448098
http://dx.doi.org/10.12688/f1000research.17964.1
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