Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows

BACKGROUND: Genetic polymorphisms are known to influence milk production and composition. However, the genomic mechanisms involved in the genetic regulation of milk component synthesis are not completely understood. MicroRNAs (miRNAs) regulate gene expression. Previous research suggests that the hig...

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Autores principales: Billa, P. A., Faulconnier, Y., Ye, T., Chervet, M., Le Provost, F., Pires, J. A. A., Leroux, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668132/
https://www.ncbi.nlm.nih.gov/pubmed/31362707
http://dx.doi.org/10.1186/s12864-019-5987-4
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author Billa, P. A.
Faulconnier, Y.
Ye, T.
Chervet, M.
Le Provost, F.
Pires, J. A. A.
Leroux, C.
author_facet Billa, P. A.
Faulconnier, Y.
Ye, T.
Chervet, M.
Le Provost, F.
Pires, J. A. A.
Leroux, C.
author_sort Billa, P. A.
collection PubMed
description BACKGROUND: Genetic polymorphisms are known to influence milk production and composition. However, the genomic mechanisms involved in the genetic regulation of milk component synthesis are not completely understood. MicroRNAs (miRNAs) regulate gene expression. Previous research suggests that the high developmental potential of the mammary gland may depend in part on a specific miRNA expression pattern. The objective of the present study was to compare the mammary gland miRNomes of two dairy cow breeds, Holstein and Montbéliarde, which have different mammogenic potentials that are related to differences in dairy performance. RESULTS: Milk, fat, protein, and lactose yields were lower in Montbéliarde cows than in Holstein cows. We detected 754 distinct miRNAs in the mammary glands of Holstein (n = 5) and Montbéliarde (n = 6) midlactating cows using RNA-Seq technology, among which 738 were known and 16 were predicted miRNAs. The 25 most abundant miRNAs accounted for 90.6% of the total reads. The comparison of their abundances in the mammary glands of Holstein versus Montbéliarde cows identified 22 differentially expressed miRNAs (P(adj) ≤ 0.05). Among them, 11 presented a fold change ≥2, and 2 (miR-100 and miR-146b) were highly expressed. Among the most abundant miRNAs, miR-186 is known to inhibit cell proliferation and epithelial-to-mesenchymal transition. Data mining showed that 17 differentially expressed miRNAs with more than 20 reads were involved in the regulation of mammary gland plasticity. Several of them may potentially target mRNAs involved in signaling pathways (such as mTOR) and lipid metabolism, thereby indicating that they could influence milk composition. CONCLUSION: We found differences in the mammary gland miRNomes of two dairy cattle breeds. These differences suggest a potential role for miRNAs in mammary gland plasticity and milk component synthesis, both of which are related to milk production and composition. Further research is warranted on the genetic regulation of miRNAs and their role in milk synthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5987-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-66681322019-08-05 Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows Billa, P. A. Faulconnier, Y. Ye, T. Chervet, M. Le Provost, F. Pires, J. A. A. Leroux, C. BMC Genomics Research Article BACKGROUND: Genetic polymorphisms are known to influence milk production and composition. However, the genomic mechanisms involved in the genetic regulation of milk component synthesis are not completely understood. MicroRNAs (miRNAs) regulate gene expression. Previous research suggests that the high developmental potential of the mammary gland may depend in part on a specific miRNA expression pattern. The objective of the present study was to compare the mammary gland miRNomes of two dairy cow breeds, Holstein and Montbéliarde, which have different mammogenic potentials that are related to differences in dairy performance. RESULTS: Milk, fat, protein, and lactose yields were lower in Montbéliarde cows than in Holstein cows. We detected 754 distinct miRNAs in the mammary glands of Holstein (n = 5) and Montbéliarde (n = 6) midlactating cows using RNA-Seq technology, among which 738 were known and 16 were predicted miRNAs. The 25 most abundant miRNAs accounted for 90.6% of the total reads. The comparison of their abundances in the mammary glands of Holstein versus Montbéliarde cows identified 22 differentially expressed miRNAs (P(adj) ≤ 0.05). Among them, 11 presented a fold change ≥2, and 2 (miR-100 and miR-146b) were highly expressed. Among the most abundant miRNAs, miR-186 is known to inhibit cell proliferation and epithelial-to-mesenchymal transition. Data mining showed that 17 differentially expressed miRNAs with more than 20 reads were involved in the regulation of mammary gland plasticity. Several of them may potentially target mRNAs involved in signaling pathways (such as mTOR) and lipid metabolism, thereby indicating that they could influence milk composition. CONCLUSION: We found differences in the mammary gland miRNomes of two dairy cattle breeds. These differences suggest a potential role for miRNAs in mammary gland plasticity and milk component synthesis, both of which are related to milk production and composition. Further research is warranted on the genetic regulation of miRNAs and their role in milk synthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5987-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-30 /pmc/articles/PMC6668132/ /pubmed/31362707 http://dx.doi.org/10.1186/s12864-019-5987-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Billa, P. A.
Faulconnier, Y.
Ye, T.
Chervet, M.
Le Provost, F.
Pires, J. A. A.
Leroux, C.
Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title_full Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title_fullStr Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title_full_unstemmed Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title_short Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows
title_sort deep rna-seq reveals mirnome differences in mammary tissue of lactating holstein and montbéliarde cows
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668132/
https://www.ncbi.nlm.nih.gov/pubmed/31362707
http://dx.doi.org/10.1186/s12864-019-5987-4
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