Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays

Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To th...

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Autores principales: Tomar, Prabhat Pratap Singh, Oren, Rivka, Krugliak, Miriam, Arkin, Isaiah T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669592/
https://www.ncbi.nlm.nih.gov/pubmed/31324045
http://dx.doi.org/10.3390/v11070632
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author Tomar, Prabhat Pratap Singh
Oren, Rivka
Krugliak, Miriam
Arkin, Isaiah T.
author_facet Tomar, Prabhat Pratap Singh
Oren, Rivka
Krugliak, Miriam
Arkin, Isaiah T.
author_sort Tomar, Prabhat Pratap Singh
collection PubMed
description Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To this end, using three bacterial assays we analyzed six small hydrophobic proteins from biomedically important viruses as potential viroporin candidates. Our results indicate that Eastern equine encephalitis virus 6k, West Nile virus MgM, Dengue virus 2k, Dengue virus P1, Variola virus gp170, and Variola virus gp151 proteins all exhibit channel activity in the bacterial assays, and as such may be considered viroporin candidates. It is clear that more studies, such as patch clamping, will be needed to characterize the ionic conductivities of these proteins. However, our approach presents a rapid procedure to analyze open reading frames in other viruses, yielding new viroporin candidates for future detailed investigation. Finally, if conductivity is proven vital to their cognate viruses, the bio-assays presented herein afford a simple approach to screen for new channel blockers.
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spelling pubmed-66695922019-08-08 Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays Tomar, Prabhat Pratap Singh Oren, Rivka Krugliak, Miriam Arkin, Isaiah T. Viruses Article Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To this end, using three bacterial assays we analyzed six small hydrophobic proteins from biomedically important viruses as potential viroporin candidates. Our results indicate that Eastern equine encephalitis virus 6k, West Nile virus MgM, Dengue virus 2k, Dengue virus P1, Variola virus gp170, and Variola virus gp151 proteins all exhibit channel activity in the bacterial assays, and as such may be considered viroporin candidates. It is clear that more studies, such as patch clamping, will be needed to characterize the ionic conductivities of these proteins. However, our approach presents a rapid procedure to analyze open reading frames in other viruses, yielding new viroporin candidates for future detailed investigation. Finally, if conductivity is proven vital to their cognate viruses, the bio-assays presented herein afford a simple approach to screen for new channel blockers. MDPI 2019-07-09 /pmc/articles/PMC6669592/ /pubmed/31324045 http://dx.doi.org/10.3390/v11070632 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomar, Prabhat Pratap Singh
Oren, Rivka
Krugliak, Miriam
Arkin, Isaiah T.
Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title_full Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title_fullStr Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title_full_unstemmed Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title_short Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
title_sort potential viroporin candidates from pathogenic viruses using bacteria-based bioassays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669592/
https://www.ncbi.nlm.nih.gov/pubmed/31324045
http://dx.doi.org/10.3390/v11070632
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