Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays
Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669592/ https://www.ncbi.nlm.nih.gov/pubmed/31324045 http://dx.doi.org/10.3390/v11070632 |
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author | Tomar, Prabhat Pratap Singh Oren, Rivka Krugliak, Miriam Arkin, Isaiah T. |
author_facet | Tomar, Prabhat Pratap Singh Oren, Rivka Krugliak, Miriam Arkin, Isaiah T. |
author_sort | Tomar, Prabhat Pratap Singh |
collection | PubMed |
description | Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To this end, using three bacterial assays we analyzed six small hydrophobic proteins from biomedically important viruses as potential viroporin candidates. Our results indicate that Eastern equine encephalitis virus 6k, West Nile virus MgM, Dengue virus 2k, Dengue virus P1, Variola virus gp170, and Variola virus gp151 proteins all exhibit channel activity in the bacterial assays, and as such may be considered viroporin candidates. It is clear that more studies, such as patch clamping, will be needed to characterize the ionic conductivities of these proteins. However, our approach presents a rapid procedure to analyze open reading frames in other viruses, yielding new viroporin candidates for future detailed investigation. Finally, if conductivity is proven vital to their cognate viruses, the bio-assays presented herein afford a simple approach to screen for new channel blockers. |
format | Online Article Text |
id | pubmed-6669592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66695922019-08-08 Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays Tomar, Prabhat Pratap Singh Oren, Rivka Krugliak, Miriam Arkin, Isaiah T. Viruses Article Viroporins are a family of small hydrophobic proteins found in many enveloped viruses that are capable of ion transport. Building upon the ability to inhibit influenza by blocking its archetypical M2 H(+) channel, as a family, viroporins may represent a viable target to curb viral infectivity. To this end, using three bacterial assays we analyzed six small hydrophobic proteins from biomedically important viruses as potential viroporin candidates. Our results indicate that Eastern equine encephalitis virus 6k, West Nile virus MgM, Dengue virus 2k, Dengue virus P1, Variola virus gp170, and Variola virus gp151 proteins all exhibit channel activity in the bacterial assays, and as such may be considered viroporin candidates. It is clear that more studies, such as patch clamping, will be needed to characterize the ionic conductivities of these proteins. However, our approach presents a rapid procedure to analyze open reading frames in other viruses, yielding new viroporin candidates for future detailed investigation. Finally, if conductivity is proven vital to their cognate viruses, the bio-assays presented herein afford a simple approach to screen for new channel blockers. MDPI 2019-07-09 /pmc/articles/PMC6669592/ /pubmed/31324045 http://dx.doi.org/10.3390/v11070632 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tomar, Prabhat Pratap Singh Oren, Rivka Krugliak, Miriam Arkin, Isaiah T. Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title | Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title_full | Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title_fullStr | Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title_full_unstemmed | Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title_short | Potential Viroporin Candidates From Pathogenic Viruses Using Bacteria-Based Bioassays |
title_sort | potential viroporin candidates from pathogenic viruses using bacteria-based bioassays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669592/ https://www.ncbi.nlm.nih.gov/pubmed/31324045 http://dx.doi.org/10.3390/v11070632 |
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