Cargando…

Episodic evolution of a eukaryotic NADK repertoire of ancient provenance

NAD kinase (NADK) is the sole enzyme that phosphorylates nicotinamide adenine dinucleotide (NAD+/NADH) into NADP+/NADPH, which provides the chemical reducing power in anabolic (biosynthetic) pathways. While prokaryotes typically encode a single NADK, eukaryotes encode multiple NADKs. How these diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Vickman, Oliver, Erives, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675116/
https://www.ncbi.nlm.nih.gov/pubmed/31369599
http://dx.doi.org/10.1371/journal.pone.0220447
_version_ 1783440620732284928
author Vickman, Oliver
Erives, Albert
author_facet Vickman, Oliver
Erives, Albert
author_sort Vickman, Oliver
collection PubMed
description NAD kinase (NADK) is the sole enzyme that phosphorylates nicotinamide adenine dinucleotide (NAD+/NADH) into NADP+/NADPH, which provides the chemical reducing power in anabolic (biosynthetic) pathways. While prokaryotes typically encode a single NADK, eukaryotes encode multiple NADKs. How these different NADK genes are all related to each other and those of prokaryotes is not known. Here we conduct phylogenetic analysis of NADK genes and identify major clade-defining patterns of NADK evolution. First, almost all eukaryotic NADK genes belong to one of two ancient eukaryotic sister clades corresponding to cytosolic (“cyto”) and mitochondrial (“mito”) clades. Secondly, we find that the cyto-clade NADK gene is duplicated in connection with loss of the mito-clade NADK gene in several eukaryotic clades or with acquisition of plastids in Archaeplastida. Thirdly, we find that horizontal gene transfers from proteobacteria have replaced mitochondrial NADK genes in only a few rare cases. Last, we find that the eukaryotic cyto and mito paralogs are unrelated to independent duplications that occurred in sporulating bacteria, once in mycelial Actinobacteria and once in aerobic endospore-forming Firmicutes. Altogether these findings show that the eukaryotic NADK gene repertoire is ancient and evolves episodically with major evolutionary transitions.
format Online
Article
Text
id pubmed-6675116
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-66751162019-08-06 Episodic evolution of a eukaryotic NADK repertoire of ancient provenance Vickman, Oliver Erives, Albert PLoS One Research Article NAD kinase (NADK) is the sole enzyme that phosphorylates nicotinamide adenine dinucleotide (NAD+/NADH) into NADP+/NADPH, which provides the chemical reducing power in anabolic (biosynthetic) pathways. While prokaryotes typically encode a single NADK, eukaryotes encode multiple NADKs. How these different NADK genes are all related to each other and those of prokaryotes is not known. Here we conduct phylogenetic analysis of NADK genes and identify major clade-defining patterns of NADK evolution. First, almost all eukaryotic NADK genes belong to one of two ancient eukaryotic sister clades corresponding to cytosolic (“cyto”) and mitochondrial (“mito”) clades. Secondly, we find that the cyto-clade NADK gene is duplicated in connection with loss of the mito-clade NADK gene in several eukaryotic clades or with acquisition of plastids in Archaeplastida. Thirdly, we find that horizontal gene transfers from proteobacteria have replaced mitochondrial NADK genes in only a few rare cases. Last, we find that the eukaryotic cyto and mito paralogs are unrelated to independent duplications that occurred in sporulating bacteria, once in mycelial Actinobacteria and once in aerobic endospore-forming Firmicutes. Altogether these findings show that the eukaryotic NADK gene repertoire is ancient and evolves episodically with major evolutionary transitions. Public Library of Science 2019-08-01 /pmc/articles/PMC6675116/ /pubmed/31369599 http://dx.doi.org/10.1371/journal.pone.0220447 Text en © 2019 Vickman, Erives http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vickman, Oliver
Erives, Albert
Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title_full Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title_fullStr Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title_full_unstemmed Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title_short Episodic evolution of a eukaryotic NADK repertoire of ancient provenance
title_sort episodic evolution of a eukaryotic nadk repertoire of ancient provenance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675116/
https://www.ncbi.nlm.nih.gov/pubmed/31369599
http://dx.doi.org/10.1371/journal.pone.0220447
work_keys_str_mv AT vickmanoliver episodicevolutionofaeukaryoticnadkrepertoireofancientprovenance
AT erivesalbert episodicevolutionofaeukaryoticnadkrepertoireofancientprovenance