A portable epigenetic switch for bistable gene expression in bacteria

We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON...

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Autores principales: Olivenza, David R., Nicoloff, Hervé, Sánchez-Romero, María Antonia, Cota, Ignacio, Andersson, Dan I., Casadesús, Josep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677893/
https://www.ncbi.nlm.nih.gov/pubmed/31375711
http://dx.doi.org/10.1038/s41598-019-47650-2
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author Olivenza, David R.
Nicoloff, Hervé
Sánchez-Romero, María Antonia
Cota, Ignacio
Andersson, Dan I.
Casadesús, Josep
author_facet Olivenza, David R.
Nicoloff, Hervé
Sánchez-Romero, María Antonia
Cota, Ignacio
Andersson, Dan I.
Casadesús, Josep
author_sort Olivenza, David R.
collection PubMed
description We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.
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spelling pubmed-66778932019-08-08 A portable epigenetic switch for bistable gene expression in bacteria Olivenza, David R. Nicoloff, Hervé Sánchez-Romero, María Antonia Cota, Ignacio Andersson, Dan I. Casadesús, Josep Sci Rep Article We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics. Nature Publishing Group UK 2019-08-02 /pmc/articles/PMC6677893/ /pubmed/31375711 http://dx.doi.org/10.1038/s41598-019-47650-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Olivenza, David R.
Nicoloff, Hervé
Sánchez-Romero, María Antonia
Cota, Ignacio
Andersson, Dan I.
Casadesús, Josep
A portable epigenetic switch for bistable gene expression in bacteria
title A portable epigenetic switch for bistable gene expression in bacteria
title_full A portable epigenetic switch for bistable gene expression in bacteria
title_fullStr A portable epigenetic switch for bistable gene expression in bacteria
title_full_unstemmed A portable epigenetic switch for bistable gene expression in bacteria
title_short A portable epigenetic switch for bistable gene expression in bacteria
title_sort portable epigenetic switch for bistable gene expression in bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677893/
https://www.ncbi.nlm.nih.gov/pubmed/31375711
http://dx.doi.org/10.1038/s41598-019-47650-2
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