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Single-Cell Point Constrictions for Reagent-Free High-Throughput Mechanical Lysis and Intact Nuclei Isolation

Highly localized (point) constrictions featuring a round geometry with ultra-sharp edges in silicon have been demonstrated for the reagent-free continuous-flow rapid mechanical lysis of mammalian cells on a single-cell basis. Silicon point constrictions, robust structures formed by a single-step dry...

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Detalles Bibliográficos
Autores principales: Huang, Xiaomin, Xing, Xiaoxing, Ng, Chun Ning, Yobas, Levent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680784/
https://www.ncbi.nlm.nih.gov/pubmed/31331049
http://dx.doi.org/10.3390/mi10070488