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Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content

Research on plant antioxidants, such as ascorbic acid (AsA) and polyphenols, is of increasing interest in plant science because of the health benefits and preventive role in chronic diseases of these natural compounds. Pepper (Capiscum annuum L.) is a major dietary source of antioxidants, especially...

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Autores principales: Chiaiese, Pasquale, Corrado, Giandomenico, Minutolo, Maria, Barone, Amalia, Errico, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681188/
https://www.ncbi.nlm.nih.gov/pubmed/31277433
http://dx.doi.org/10.3390/plants8070206
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author Chiaiese, Pasquale
Corrado, Giandomenico
Minutolo, Maria
Barone, Amalia
Errico, Angela
author_facet Chiaiese, Pasquale
Corrado, Giandomenico
Minutolo, Maria
Barone, Amalia
Errico, Angela
author_sort Chiaiese, Pasquale
collection PubMed
description Research on plant antioxidants, such as ascorbic acid (AsA) and polyphenols, is of increasing interest in plant science because of the health benefits and preventive role in chronic diseases of these natural compounds. Pepper (Capiscum annuum L.) is a major dietary source of antioxidants, especially AsA. Although considerable advance has been made, our understanding of AsA biosynthesis and its regulation in higher plants is not yet exhaustive. For instance, while it is accepted that AsA content in cells is regulated at different levels (e.g., transcriptional and post-transcriptional), their relative prominence is not fully understood. In this work, we identified and studied two pepper varieties with low and high levels of AsA to shed light on the transcriptional mechanisms that can account for the observed phenotypes. We quantified AsA and polyphenols in leaves and during fruit maturation, and concurrently, we analyzed the transcription of 14 genes involved in AsA biosynthesis, degradation, and recycling. The differential transcriptional analysis indicated that the higher expression of genes involved in AsA accumulation is a likely explanation for the observed differences in fruits. This was also supported by the identification of gene-metabolite relations, which deserve further investigation. Our results provide new insights into AsA differential accumulation in pepper varieties and highlight the phenotypic diversity in local germplasm, a knowledge that may ultimately contribute to the increased level of health-related phytochemicals.
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spelling pubmed-66811882019-08-09 Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content Chiaiese, Pasquale Corrado, Giandomenico Minutolo, Maria Barone, Amalia Errico, Angela Plants (Basel) Article Research on plant antioxidants, such as ascorbic acid (AsA) and polyphenols, is of increasing interest in plant science because of the health benefits and preventive role in chronic diseases of these natural compounds. Pepper (Capiscum annuum L.) is a major dietary source of antioxidants, especially AsA. Although considerable advance has been made, our understanding of AsA biosynthesis and its regulation in higher plants is not yet exhaustive. For instance, while it is accepted that AsA content in cells is regulated at different levels (e.g., transcriptional and post-transcriptional), their relative prominence is not fully understood. In this work, we identified and studied two pepper varieties with low and high levels of AsA to shed light on the transcriptional mechanisms that can account for the observed phenotypes. We quantified AsA and polyphenols in leaves and during fruit maturation, and concurrently, we analyzed the transcription of 14 genes involved in AsA biosynthesis, degradation, and recycling. The differential transcriptional analysis indicated that the higher expression of genes involved in AsA accumulation is a likely explanation for the observed differences in fruits. This was also supported by the identification of gene-metabolite relations, which deserve further investigation. Our results provide new insights into AsA differential accumulation in pepper varieties and highlight the phenotypic diversity in local germplasm, a knowledge that may ultimately contribute to the increased level of health-related phytochemicals. MDPI 2019-07-04 /pmc/articles/PMC6681188/ /pubmed/31277433 http://dx.doi.org/10.3390/plants8070206 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiaiese, Pasquale
Corrado, Giandomenico
Minutolo, Maria
Barone, Amalia
Errico, Angela
Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title_full Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title_fullStr Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title_full_unstemmed Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title_short Transcriptional Regulation of Ascorbic Acid During Fruit Ripening in Pepper (Capsicum annuum) Varieties with Low and High Antioxidants Content
title_sort transcriptional regulation of ascorbic acid during fruit ripening in pepper (capsicum annuum) varieties with low and high antioxidants content
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681188/
https://www.ncbi.nlm.nih.gov/pubmed/31277433
http://dx.doi.org/10.3390/plants8070206
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