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CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida

Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking conspicuou...

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Detalles Bibliográficos
Autores principales: Aparicio, Tomás, de Lorenzo, Víctor, Martínez‐García, Esteban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681395/
https://www.ncbi.nlm.nih.gov/pubmed/31237429
http://dx.doi.org/10.1111/1751-7915.13453