Cargando…

CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida

Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking conspicuou...

Descripción completa

Detalles Bibliográficos
Autores principales:	Aparicio, Tomás, de Lorenzo, Víctor, Martínez‐García, Esteban
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	Brief Reports
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681395/ https://www.ncbi.nlm.nih.gov/pubmed/31237429 http://dx.doi.org/10.1111/1751-7915.13453

Ejemplares similares

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering
por: Aparicio, Tomas, et al.
Publicado: (2020)

Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection
por: Wirth, Nicolas T., et al.
Publicado: (2019)

CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish
por: Bai, Haipeng, et al.
Publicado: (2020)

An automated DIY framework for experimental evolution of Pseudomonas putida
por: Espeso, David R., et al.
Publicado: (2020)

CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
por: Marino, Nicole D, et al.
Publicado: (2022)

Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor
por: Ranawakage, Deshani C., et al.
Publicado: (2021)

ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
por: Al-ramahi, Yamal, et al.
Publicado: (2021)

CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping
por: Marinov, Georgi K., et al.
Publicado: (2023)

RPA homologs and ssDNA processing during meiotic recombination
por: Ribeiro, Jonathan, et al.
Publicado: (2015)

Details of ssDNA annealing revealed by an HSV-1 ICP8–ssDNA binary complex
por: Tolun, Gökhan, et al.
Publicado: (2013)

CRISPR–Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5′ ssDNA exonucleases
por: Radovčić, Marin, et al.
Publicado: (2018)

Dual functions for the ssDNA-binding protein RPA in meiotic recombination
por: Shi, Baolu, et al.
Publicado: (2019)

CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440
por: Kim, Seong Keun, et al.
Publicado: (2019)

Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos
por: Wei, Yu, et al.
Publicado: (2021)

Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA
por: Khil, Pavel P., et al.
Publicado: (2012)

The Borrelia burgdorferi telomere resolvase, ResT, anneals ssDNA complexed with its cognate ssDNA-binding protein
por: Huang, Shu Hui, et al.
Publicado: (2016)

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression
por: Martínez-García, Esteban, et al.
Publicado: (2014)

Mechanochemical regulations of RPA's binding to ssDNA
por: Chen, Jin, et al.
Publicado: (2015)

Acousto-microfluidics for screening of ssDNA aptamer
por: Park, Jee-Woong, et al.
Publicado: (2016)

Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
por: Liu, Jin, et al.
Publicado: (2021)

Structure, stability and specificity of the binding of ssDNA and ssRNA with proteins
por: Pal, Arumay, et al.
Publicado: (2019)

Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri
por: van Pijkeren, Jan-Peter, et al.
Publicado: (2012)

Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies
por: Fraile, Sofía, et al.
Publicado: (2021)

Gross transcriptomic analysis of Pseudomonas putida for diagnosing environmental shifts
por: Hueso‐Gil, Ángeles, et al.
Publicado: (2019)

A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity
por: He, Liu, et al.
Publicado: (2021)

Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma
por: Parekh, Parag, et al.
Publicado: (2013)

A comparative study of protein–ssDNA interactions
por: Lin, Maoxuan, et al.
Publicado: (2021)

Targetron-Assisted Delivery of Exogenous DNA Sequences into Pseudomonas putida through CRISPR-Aided Counterselection
por: Velázquez, Elena, et al.
Publicado: (2021)

The ssDNA-binding protein MEIOB acts as a dosage-sensitive regulator of meiotic recombination
por: Guo, Rui, et al.
Publicado: (2020)

Pressure‐dependent growth controls 3D architecture of Pseudomonas putida microcolonies
por: Kim, Juhyun, et al.
Publicado: (2023)

Probing the mechanism of recognition of ssDNA by the Cdc13-DBD
por: Eldridge, Aimee M., et al.
Publicado: (2008)

Intrinsic Stepwise Translocation of Stretched ssDNA in Graphene Nanopores
por: Qiu, Hu, et al.
Publicado: (2015)

Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex
por: Witosch, Justine, et al.
Publicado: (2014)

Screening and Identification of ssDNA Aptamer for Human GP73
por: Du, Jingchun, et al.
Publicado: (2015)

Selection and targeting of EpCAM protein by ssDNA aptamer
por: Alshaer, Walhan, et al.
Publicado: (2017)

Selection, Characterization, and Application of ssDNA Aptamer against Furaneol
por: Komarova, Natalia, et al.
Publicado: (2018)

DSS1 and ssDNA regulate oligomerization of BRCA2
por: Le, Hang Phuong, et al.
Publicado: (2020)

Selection and identification of an ssDNA aptamer to NB4 cell
por: Zhang, Xian‐hui, et al.
Publicado: (2021)

Selection and Identification of an ssDNA Aptamer for Fibroblast Activation Protein
por: Zhang, Xiaomin, et al.
Publicado: (2023)

Dna2 removes toxic ssDNA-RPA filaments generated from meiotic recombination-associated DNA synthesis
por: Zhai, Binyuan, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_arfl4gi38i8n4r0fre1a5iqc2c