Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry

[Image: see text] Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G(4)Y), mono-functionalization of the monoclonal antibody trastuz...

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Autores principales: Bruins, Jorick J., van de Wouw, Criss, Wagner, Koen, Bartels, Lina, Albada, Bauke, van Delft, Floris L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682001/
https://www.ncbi.nlm.nih.gov/pubmed/31460288
http://dx.doi.org/10.1021/acsomega.9b01727
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author Bruins, Jorick J.
van de Wouw, Criss
Wagner, Koen
Bartels, Lina
Albada, Bauke
van Delft, Floris L.
author_facet Bruins, Jorick J.
van de Wouw, Criss
Wagner, Koen
Bartels, Lina
Albada, Bauke
van Delft, Floris L.
author_sort Bruins, Jorick J.
collection PubMed
description [Image: see text] Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G(4)Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.
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spelling pubmed-66820012019-08-27 Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry Bruins, Jorick J. van de Wouw, Criss Wagner, Koen Bartels, Lina Albada, Bauke van Delft, Floris L. ACS Omega [Image: see text] Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G(4)Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab. American Chemical Society 2019-07-08 /pmc/articles/PMC6682001/ /pubmed/31460288 http://dx.doi.org/10.1021/acsomega.9b01727 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Bruins, Jorick J.
van de Wouw, Criss
Wagner, Koen
Bartels, Lina
Albada, Bauke
van Delft, Floris L.
Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title_full Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title_fullStr Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title_full_unstemmed Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title_short Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
title_sort highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682001/
https://www.ncbi.nlm.nih.gov/pubmed/31460288
http://dx.doi.org/10.1021/acsomega.9b01727
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