Enhanced Activity and Substrate Specificity by Site‐Directed Mutagenesis for the P450 119 Peroxygenase Catalyzed Sulfoxidation of Thioanisole

P450 119 peroxygenase was found to catalyze the sulfoxidation of thioanisole and the sulfonation of sulfoxide in the presence of tert‐butyl hydroperoxide (TBHP) for the first time with turnover rates of 1549 min(−1) and 196 min(−1) respectively. Several mutants were designed to improve the peroxygenation activity and thioanisole specificity by site‐directed mutagenesis. The F153G/T213G mutant gave an increase of sulfoxide yield and a decrease of sulfone yield. Moreover the S148P/I161T/K199E/T214V mutant and the K199E mutant with acidic Glu residue contributed to improving the product ratio of sulfoxide to sulfone. Addition of short‐alkyl‐chain organic acids to the P450 119 peroxygenase‐catalyzed sulfur oxidation of thioanisole was investigated. Octanoic acid was found to induce a preferred sulfoxidation of thioanisole catalyzed by the F153G/T213G mutant to give approximately 2.4‐fold increase in turnover rate with a k (cat) value of 3687 min(−1) relative to that of the wild‐type, and by the F153G mutant to give the R‐sulfoxide up to 30 % ee. The experimental control and the proposed mechanism for the P450 119 peroxygenase‐catalyzed sulfoxidation of thioanisole in the presence of octanoic acid suggested that octanoic acid could partially occupy the substrate pocket; meanwhile the F153G mutation could enhance the substrate specificity, which could lead to efficiently regulate the spatial orientation of thioanisole and facilitate the formation of Compound I. This is the most effective catalytic system for the P450 119 peroxygenase‐catalyzed sulfoxidation of thioanisole.