Visualisation of dCas9 target search in vivo using an open-microscopy framework

CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its relation to the cellular concentration of Cas9 have remained elusive. Effective target search requires constant screening of the protospacer adjacent motif (PAM) and a 30 ms upper limit for screening was recentl...

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Detalles Bibliográficos
Autores principales: Martens, Koen J. A., van Beljouw, Sam P. B., van der Els, Simon, Vink, Jochem N. A., Baas, Sander, Vogelaar, George A., Brouns, Stan J. J., van Baarlen, Peter, Kleerebezem, Michiel, Hohlbein, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6685946/
https://www.ncbi.nlm.nih.gov/pubmed/31391532
http://dx.doi.org/10.1038/s41467-019-11514-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6685946/
https://www.ncbi.nlm.nih.gov/pubmed/31391532
http://dx.doi.org/10.1038/s41467-019-11514-0

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