Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization

The isolation of high‐quality RNA from the intervertebral disc and especially from the nucleus pulposus is challenging due to the low cellularity and high proteoglycan content of this tissue. In this study, we report a simple modification of the standard guanidinium thiocyanate‐phenol‐chloroform ext...

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Detalles Bibliográficos
Autores principales: Caprez, Stephanie, Menzel, Ursula, Li, Zhen, Grad, Sibylle, Alini, Mauro, Peroglio, Marianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686795/
https://www.ncbi.nlm.nih.gov/pubmed/31463444
http://dx.doi.org/10.1002/jsp2.1017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686795/
https://www.ncbi.nlm.nih.gov/pubmed/31463444
http://dx.doi.org/10.1002/jsp2.1017

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