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Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization
The isolation of high‐quality RNA from the intervertebral disc and especially from the nucleus pulposus is challenging due to the low cellularity and high proteoglycan content of this tissue. In this study, we report a simple modification of the standard guanidinium thiocyanate‐phenol‐chloroform ext...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686795/ https://www.ncbi.nlm.nih.gov/pubmed/31463444 http://dx.doi.org/10.1002/jsp2.1017 |