Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background

Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces cerevisiae) to incorporate a new genetic pathway in place of a native pathway – even an essential one – while maintaining low false positive rates (less than 1 in 10(8) per cell). This plasmid, dubbed “Superloser,”...

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Detalles Bibliográficos
Autores principales: Haase, Max A. B., Truong, David M., Boeke, Jef D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686923/
https://www.ncbi.nlm.nih.gov/pubmed/31213518
http://dx.doi.org/10.1534/g3.119.400325

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686923/
https://www.ncbi.nlm.nih.gov/pubmed/31213518
http://dx.doi.org/10.1534/g3.119.400325

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