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Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background
Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces cerevisiae) to incorporate a new genetic pathway in place of a native pathway – even an essential one – while maintaining low false positive rates (less than 1 in 10(8) per cell). This plasmid, dubbed “Superloser,”...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686923/ https://www.ncbi.nlm.nih.gov/pubmed/31213518 http://dx.doi.org/10.1534/g3.119.400325 |