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Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans

The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans. Here, we have streamlined the process by creating a rapid, cloning-free method of single-copy transgene insertion we call Mos1 el...

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Detalles Bibliográficos
Autores principales: Philip, Nicholas S., Escobedo, Fernando, Bahr, Laura L., Berry, Brandon J., Wojtovich, Andrew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686933/
https://www.ncbi.nlm.nih.gov/pubmed/31186306
http://dx.doi.org/10.1534/g3.119.400399