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Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans
The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans. Here, we have streamlined the process by creating a rapid, cloning-free method of single-copy transgene insertion we call Mos1 el...
Autores principales: | Philip, Nicholas S., Escobedo, Fernando, Bahr, Laura L., Berry, Brandon J., Wojtovich, Andrew P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686933/ https://www.ncbi.nlm.nih.gov/pubmed/31186306 http://dx.doi.org/10.1534/g3.119.400399 |
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