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Detection and characterization of a theta-replicating plasmid pLP60 from Lactobacillus plantarum PC518 by inverse PCR

Plasmid DNA of Lactobacillus plantarum PC518 was isolated by an improved method which contained a washing step for removing lysozyme. Three plasmid DNA libraries were constructed. A pair of outward primers was designed at both ends of the novel plasmid fragment obtained from plasmid DNA libraries, a...

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Detalles Bibliográficos
Autores principales: Yao, Fang, Xu, XiaoYu, Du, Xin, Cao, Kang, Pan, Qu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687099/
https://www.ncbi.nlm.nih.gov/pubmed/31414068
http://dx.doi.org/10.1016/j.heliyon.2019.e02164