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Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set

Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a multi-species primer set to validate referenc...

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Detalles Bibliográficos
Autores principales: Moura, Marcelo T., Silva, Roberta L. O., Nascimento, Pábola S., Ferreira-Silva, José C., Cantanhêde, Ludymila F., Kido, Ederson A., Benko-Iseppon, Ana M., Oliveira, Marcos A. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693880/
https://www.ncbi.nlm.nih.gov/pubmed/31412093
http://dx.doi.org/10.1371/journal.pone.0221170