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Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set
Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a multi-species primer set to validate referenc...
Autores principales: | Moura, Marcelo T., Silva, Roberta L. O., Nascimento, Pábola S., Ferreira-Silva, José C., Cantanhêde, Ludymila F., Kido, Ederson A., Benko-Iseppon, Ana M., Oliveira, Marcos A. L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693880/ https://www.ncbi.nlm.nih.gov/pubmed/31412093 http://dx.doi.org/10.1371/journal.pone.0221170 |
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