Cargando…
SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS
A multi-class wide-scope screening method for the detection and identification of artificial colours and illegal dyes in spices was developed for regulatory purposes. The screening was carried out by ultra-high performance liquid chromatography hyphenated with a quadrupole/time-of-flight mass spectr...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694849/ https://www.ncbi.nlm.nih.gov/pubmed/31432009 http://dx.doi.org/10.1016/j.fochx.2019.100009 |
_version_ | 1783443910417186816 |
---|---|
author | Périat, Aurélie Bieri, Stefan Mottier, Nicolas |
author_facet | Périat, Aurélie Bieri, Stefan Mottier, Nicolas |
author_sort | Périat, Aurélie |
collection | PubMed |
description | A multi-class wide-scope screening method for the detection and identification of artificial colours and illegal dyes in spices was developed for regulatory purposes. The screening was carried out by ultra-high performance liquid chromatography hyphenated with a quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS) with sequential window acquisition of all theoretical fragment-ion spectra (SWATH) and was validated with forty-one compounds by spiking experiments in curry and paprika extracts. In order to detect and identify the compounds with a high level of confidence, a home-made tandem mass spectrometry (QTOF-MS/MS) database of approximately one hundred illegal dyes and artificial colours was created. The procedure was then used to screen field samples of spices and spice blends purchased from Swiss markets. Sudan IV, Sudan I, bixin (E160b) and Ponceau 4R (E124) were all detected among the eight non-compliant samples. |
format | Online Article Text |
id | pubmed-6694849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-66948492019-08-20 SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS Périat, Aurélie Bieri, Stefan Mottier, Nicolas Food Chem X Article A multi-class wide-scope screening method for the detection and identification of artificial colours and illegal dyes in spices was developed for regulatory purposes. The screening was carried out by ultra-high performance liquid chromatography hyphenated with a quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS) with sequential window acquisition of all theoretical fragment-ion spectra (SWATH) and was validated with forty-one compounds by spiking experiments in curry and paprika extracts. In order to detect and identify the compounds with a high level of confidence, a home-made tandem mass spectrometry (QTOF-MS/MS) database of approximately one hundred illegal dyes and artificial colours was created. The procedure was then used to screen field samples of spices and spice blends purchased from Swiss markets. Sudan IV, Sudan I, bixin (E160b) and Ponceau 4R (E124) were all detected among the eight non-compliant samples. Elsevier 2019-03-01 /pmc/articles/PMC6694849/ /pubmed/31432009 http://dx.doi.org/10.1016/j.fochx.2019.100009 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Périat, Aurélie Bieri, Stefan Mottier, Nicolas SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title | SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title_full | SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title_fullStr | SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title_full_unstemmed | SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title_short | SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS |
title_sort | swath-ms screening strategy for the determination of food dyes in spices by uhplc-hrms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694849/ https://www.ncbi.nlm.nih.gov/pubmed/31432009 http://dx.doi.org/10.1016/j.fochx.2019.100009 |
work_keys_str_mv | AT periataurelie swathmsscreeningstrategyforthedeterminationoffooddyesinspicesbyuhplchrms AT bieristefan swathmsscreeningstrategyforthedeterminationoffooddyesinspicesbyuhplchrms AT mottiernicolas swathmsscreeningstrategyforthedeterminationoffooddyesinspicesbyuhplchrms |