Cargando…

AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks

During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy sta...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamada, Shintaro, Kamata, Taito, Nawa, Hiroyuki, Sekijima, Tsuneo, Takei, Nobuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695389/
https://www.ncbi.nlm.nih.gov/pubmed/31417118
http://dx.doi.org/10.1038/s41598-019-48172-7
_version_ 1783444028385132544
author Yamada, Shintaro
Kamata, Taito
Nawa, Hiroyuki
Sekijima, Tsuneo
Takei, Nobuyuki
author_facet Yamada, Shintaro
Kamata, Taito
Nawa, Hiroyuki
Sekijima, Tsuneo
Takei, Nobuyuki
author_sort Yamada, Shintaro
collection PubMed
description During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy status and regulates metabolism. Therefore, we examined AMPK signaling in several brain regions and peripheral tissues in hibernating chipmunk. Eukaryotic elongation factor 2 (eEF2) is a downstream target of AMPK. Phosphorylation of eEF2, indicating its inactivation, is enhanced in the cerebral cortex of hibernating chipmunks. The study indicated that the sequential regulation of AMPK-mammalian target of rapamycin complex 1-eEF2 signaling was altered and protein synthesis ability was reduced in the cerebral cortex of hibernating chipmunks.
format Online
Article
Text
id pubmed-6695389
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66953892019-08-19 AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks Yamada, Shintaro Kamata, Taito Nawa, Hiroyuki Sekijima, Tsuneo Takei, Nobuyuki Sci Rep Article During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy status and regulates metabolism. Therefore, we examined AMPK signaling in several brain regions and peripheral tissues in hibernating chipmunk. Eukaryotic elongation factor 2 (eEF2) is a downstream target of AMPK. Phosphorylation of eEF2, indicating its inactivation, is enhanced in the cerebral cortex of hibernating chipmunks. The study indicated that the sequential regulation of AMPK-mammalian target of rapamycin complex 1-eEF2 signaling was altered and protein synthesis ability was reduced in the cerebral cortex of hibernating chipmunks. Nature Publishing Group UK 2019-08-15 /pmc/articles/PMC6695389/ /pubmed/31417118 http://dx.doi.org/10.1038/s41598-019-48172-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yamada, Shintaro
Kamata, Taito
Nawa, Hiroyuki
Sekijima, Tsuneo
Takei, Nobuyuki
AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title_full AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title_fullStr AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title_full_unstemmed AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title_short AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
title_sort ampk activation, eef2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695389/
https://www.ncbi.nlm.nih.gov/pubmed/31417118
http://dx.doi.org/10.1038/s41598-019-48172-7
work_keys_str_mv AT yamadashintaro ampkactivationeef2inactivationandreducedproteinsynthesisinthecerebralcortexofhibernatingchipmunks
AT kamatataito ampkactivationeef2inactivationandreducedproteinsynthesisinthecerebralcortexofhibernatingchipmunks
AT nawahiroyuki ampkactivationeef2inactivationandreducedproteinsynthesisinthecerebralcortexofhibernatingchipmunks
AT sekijimatsuneo ampkactivationeef2inactivationandreducedproteinsynthesisinthecerebralcortexofhibernatingchipmunks
AT takeinobuyuki ampkactivationeef2inactivationandreducedproteinsynthesisinthecerebralcortexofhibernatingchipmunks