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AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy sta...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695389/ https://www.ncbi.nlm.nih.gov/pubmed/31417118 http://dx.doi.org/10.1038/s41598-019-48172-7 |
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author | Yamada, Shintaro Kamata, Taito Nawa, Hiroyuki Sekijima, Tsuneo Takei, Nobuyuki |
author_facet | Yamada, Shintaro Kamata, Taito Nawa, Hiroyuki Sekijima, Tsuneo Takei, Nobuyuki |
author_sort | Yamada, Shintaro |
collection | PubMed |
description | During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy status and regulates metabolism. Therefore, we examined AMPK signaling in several brain regions and peripheral tissues in hibernating chipmunk. Eukaryotic elongation factor 2 (eEF2) is a downstream target of AMPK. Phosphorylation of eEF2, indicating its inactivation, is enhanced in the cerebral cortex of hibernating chipmunks. The study indicated that the sequential regulation of AMPK-mammalian target of rapamycin complex 1-eEF2 signaling was altered and protein synthesis ability was reduced in the cerebral cortex of hibernating chipmunks. |
format | Online Article Text |
id | pubmed-6695389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66953892019-08-19 AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks Yamada, Shintaro Kamata, Taito Nawa, Hiroyuki Sekijima, Tsuneo Takei, Nobuyuki Sci Rep Article During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O(2) supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy status and regulates metabolism. Therefore, we examined AMPK signaling in several brain regions and peripheral tissues in hibernating chipmunk. Eukaryotic elongation factor 2 (eEF2) is a downstream target of AMPK. Phosphorylation of eEF2, indicating its inactivation, is enhanced in the cerebral cortex of hibernating chipmunks. The study indicated that the sequential regulation of AMPK-mammalian target of rapamycin complex 1-eEF2 signaling was altered and protein synthesis ability was reduced in the cerebral cortex of hibernating chipmunks. Nature Publishing Group UK 2019-08-15 /pmc/articles/PMC6695389/ /pubmed/31417118 http://dx.doi.org/10.1038/s41598-019-48172-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yamada, Shintaro Kamata, Taito Nawa, Hiroyuki Sekijima, Tsuneo Takei, Nobuyuki AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title | AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title_full | AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title_fullStr | AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title_full_unstemmed | AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title_short | AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
title_sort | ampk activation, eef2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695389/ https://www.ncbi.nlm.nih.gov/pubmed/31417118 http://dx.doi.org/10.1038/s41598-019-48172-7 |
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