Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis

AIM: To develop IL-18 peptide-based virus-like particle vaccines that elicit autoantibodies against IL-18 and to evaluate the in vivo effects of the vaccines in murine colitis. METHODS: Recombinant IL-18 vaccines were constructed, and the effects of the vaccines were evaluated in trinitrobenzene sul...

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Autores principales: Guan, Qingdong, Warrington, Richard, Moreno, Sem, Qing, Gefei, Weiss, Carolyn, Peng, Zhikang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695525/
https://www.ncbi.nlm.nih.gov/pubmed/31428451
http://dx.doi.org/10.2144/fsoa-2018-0125
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author Guan, Qingdong
Warrington, Richard
Moreno, Sem
Qing, Gefei
Weiss, Carolyn
Peng, Zhikang
author_facet Guan, Qingdong
Warrington, Richard
Moreno, Sem
Qing, Gefei
Weiss, Carolyn
Peng, Zhikang
author_sort Guan, Qingdong
collection PubMed
description AIM: To develop IL-18 peptide-based virus-like particle vaccines that elicit autoantibodies against IL-18 and to evaluate the in vivo effects of the vaccines in murine colitis. METHODS: Recombinant IL-18 vaccines were constructed, and the effects of the vaccines were evaluated in trinitrobenzene sulfonic acid-induced acute and chronic colitis in mice. RESULTS: Two murine IL-18 peptide-based vaccines (A and D) were developed, which induced relative long-lasting specific antibodies against IL-18. Vaccine-immunized mouse antisera could partially block IL-18-induced IFN-γ production in vitro. Mice receiving vaccine D, not vaccine A, had a significant decrease in intestinal inflammation, collagen deposition and pro-inflammatory cytokine levels in colon tissue. CONCLUSION: IL-18 vaccine may provide a potential therapeutic approach in the treatment of Crohn’s disease.
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spelling pubmed-66955252019-08-19 Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis Guan, Qingdong Warrington, Richard Moreno, Sem Qing, Gefei Weiss, Carolyn Peng, Zhikang Future Sci OA Short Communication AIM: To develop IL-18 peptide-based virus-like particle vaccines that elicit autoantibodies against IL-18 and to evaluate the in vivo effects of the vaccines in murine colitis. METHODS: Recombinant IL-18 vaccines were constructed, and the effects of the vaccines were evaluated in trinitrobenzene sulfonic acid-induced acute and chronic colitis in mice. RESULTS: Two murine IL-18 peptide-based vaccines (A and D) were developed, which induced relative long-lasting specific antibodies against IL-18. Vaccine-immunized mouse antisera could partially block IL-18-induced IFN-γ production in vitro. Mice receiving vaccine D, not vaccine A, had a significant decrease in intestinal inflammation, collagen deposition and pro-inflammatory cytokine levels in colon tissue. CONCLUSION: IL-18 vaccine may provide a potential therapeutic approach in the treatment of Crohn’s disease. Future Science Ltd 2019-07-30 /pmc/articles/PMC6695525/ /pubmed/31428451 http://dx.doi.org/10.2144/fsoa-2018-0125 Text en © 2019 The authors This work is licensed under the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Short Communication
Guan, Qingdong
Warrington, Richard
Moreno, Sem
Qing, Gefei
Weiss, Carolyn
Peng, Zhikang
Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title_full Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title_fullStr Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title_full_unstemmed Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title_short Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis
title_sort sustained suppression of il-18 by employing a vaccine ameliorates intestinal inflammation in tnbs-induced murine colitis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695525/
https://www.ncbi.nlm.nih.gov/pubmed/31428451
http://dx.doi.org/10.2144/fsoa-2018-0125
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